Anti-PCK2 antibody (ab40843)
Key features and details
- Goat polyclonal to PCK2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCK2 antibody
See all PCK2 primary antibodies -
Description
Goat polyclonal to PCK2 -
Host species
Goat -
Specificity
This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
KPWKPGDKEPCAH
, corresponding to Internal sequence amino acids 407-419 of Human PCK2 -
Positive control
- Human liver lysate and HepG2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PCK2 antibody (ab40843)Anti-PCK2 antibody (ab40843) at 2 µg/ml + Human Liver lysate at 35 µg/ml
Predicted band size: 71 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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Western blot - Anti-PCK2 antibody (ab40843)Anti-PCK2 antibody (ab40843) at 0.1 µg/ml + Human HepG2 lysate (35µg protein in RIPA buffer).
Predicted band size: 71 kDa
Observed band size: 71 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
