Anti-Rif1 antibody - C-terminal (ab229656)
Key features and details
- Rabbit polyclonal to Rif1 - C-terminal
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rif1 antibody - C-terminal
See all Rif1 primary antibodies -
Description
Rabbit polyclonal to Rif1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Rif1 (C terminal). The exact sequence is proprietary.
Database link: Q5UIP0 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract. IHC-P: Human breast carcinoma and cervical carcinoma tissues. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rif1 antibody - C-terminal (ab229656)All lanes : Anti-Rif1 antibody - C-terminal (ab229656) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 274 kDa5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody - C-terminal (ab229656)4% paraformaldehyde fixed heLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Rif1 (green) using ab229656 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-Rif1 antibody - C-terminal (ab229656)Rif1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab229656. Western blot was performed from the immunoprecipitate using ab229656. Anti-rabbit IgG was used as secondary antibody.
Lane 1: Control IgG IP instead of ab229656 in HeLa whole cell extract.
Lane 2: ab229656 IP in HeLa whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody - C-terminal (ab229656)Paraffin-embedded human breast carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody - C-terminal (ab229656)Paraffin-embedded human cervical carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.
