Anti-Rif1 antibody (ab229632)
Key features and details
- Rabbit polyclonal to Rif1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rif1 antibody
See all Rif1 primary antibodies -
Description
Rabbit polyclonal to Rif1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human Rif1 (internal sequence). The exact sequence is proprietary.
Database link: Q5UIP0 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rif1 antibody (ab229632)All lanes : Anti-Rif1 antibody (ab229632) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 274 kDa5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody (ab229632)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Rif1 (green) using ab229632 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue) and phalloidin, a cytoskeleton marker (red).
