Anti-CDK1 (phospho T14) antibody (ab58509)
Key features and details
- Rabbit polyclonal to CDK1 (phospho T14)
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-CDK1 (phospho T14) antibody
See all CDK1 primary antibodies -
Description
Rabbit polyclonal to CDK1 (phospho T14) -
Host species
Rabbit -
Specificity
Detects endogenous levels of CDC2 only when phosphorylated at threonine 14. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic phosphopeptide derived from human Cdc2 around the phosphorylation site of threonine 14 (E-G-TP-Y-G).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDK1 (phospho T14) antibody (ab58509)
Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM for 30 mins, using CDK1 (phospho T14) Antibody (ab58509). The lane on the right is blocked with the phospho peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-CDK1 (phospho T14) antibody (ab58509)Immunofluorescence analysis of COS7 cells using ab58509 at 1/1000 dilution.
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Western blot - Anti-CDK1 (phospho T14) antibody (ab58509)All lanes : Anti-CDK1 (phospho T14) antibody (ab58509) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins)
Lane 2 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins), plus immunising peptide
Predicted band size: 34 kDa
