Anti-GOLGA5/Golgin-84 antibody (ab224040)
Key features and details
- Rabbit polyclonal to GOLGA5/Golgin-84
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GOLGA5/Golgin-84 antibody
See all GOLGA5/Golgin-84 primary antibodies -
Description
Rabbit polyclonal to GOLGA5/Golgin-84 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human GOLGA5/Golgin-84 aa 150-350.
Database link: Q8TBA6 -
Positive control
- IHC-P: Human cerebellum, epididymis, testis and stomach tissue; WB: U-87 MG cell lysate; ICC/IF: U-2 OS cells.
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General notes
This product was previously labelled as GOLGA5
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (ab224040)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human epididymis tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (ab224040)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for GOLGA5/Golgin-84 (green) using ab224040 at 4 µg/ml in ICC/IF.
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Western blot - Anti-GOLGA5/Golgin-84 antibody (ab224040)All lanes : Anti-GOLGA5/Golgin-84 antibody (ab224040) at 0.4 µg/ml
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells transfected with control siRNA, whole cell lysate
Lane 2 : U-87MG cells transfected with target specific siRNA probe #1, whole cell lysate
Lane 3 : U-87MG cells transfected with target specific siRNA probe #2, whole cell lysate
Predicted band size: 83 kDaLoading control: GAPDH
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (ab224040)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (ab224040)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (ab224040)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (ab224040)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
