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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription TFIIF

Anti-GTF2F2 antibody [EPR7584] (ab128945)

Price and availability

237 878 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-GTF2F2 antibody [EPR7584] (ab128945)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7584] to GTF2F2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-GTF2F2 antibody [EPR7584]
    See all GTF2F2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7584] to GTF2F2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region within Human GTF2F2 (Uniprot: P13984)

  • Positive control

    • HeLa and 293T cell lysates; Human cervical carcinoma tissue; Human colon tissue
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7584
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIIF
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Preinitiation complex

Images

  • Western blot - Anti-GTF2F2 antibody [EPR7584] (ab128945)
    Western blot - Anti-GTF2F2 antibody [EPR7584] (ab128945)
    All lanes : Anti-GTF2F2 antibody [EPR7584] (ab128945) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 28 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584] (ab128945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584] (ab128945)

    ab128945 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584] (ab128945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584] (ab128945)

    ab128945 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human colon tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-GTF2F2 antibody [EPR7584] (ab128945)
    Anti-GTF2F2 antibody [EPR7584] (ab128945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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