Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53]
See all CDK1+CDK2+CDK3+CDK5 primary antibodies -
Description
Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide aa 1-100.
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Positive control
- WB: Hela cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Whole Cell Lysate -
Clonality
Monoclonal -
Clone number
E53 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/10000 dilution
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer 5% NFDM/TBST
The endogenous CDK is detected at higher exposure.
We are not sure the bands around 35kDa. Perhaps they are degradation products.
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All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/2000 dilution
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Exposure time: 15 secondsBlocking/Diluting buffer 5% NFDM/TBST
34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.
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Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/5000 dilution + Hela cell lysate
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
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