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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cancer Cell cycle Kinases/phosphatases Cdks

Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)

Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53]
    See all CDK1+CDK2+CDK3+CDK5 primary antibodies
  • Description

    Rabbit monoclonal [E53] to CDK1 + CDK2 + CDK3 + CDK5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide aa 1-100.

  • Positive control

    • WB: Hela cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Whole Cell Lysate
  • Clonality

    Monoclonal
  • Clone number

    E53
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Cdks
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Cdks
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
    All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/10000 dilution

    Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
    Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
    Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Diluting buffer 5% NFDM/TBST

    The endogenous CDK is detected at higher exposure.

    We are not sure the bands around 35kDa. Perhaps they are degradation products.

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
    All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/2000 dilution

    Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
    Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
    Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa


    Exposure time: 15 seconds


    Blocking/Diluting buffer 5% NFDM/TBST

    34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030) at 1/5000 dilution + Hela cell lysate

    Predicted band size: 34 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 antibody [E53] (ab32030)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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