Recombinant human TNF Receptor II protein (Active) (ab168884)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.
pH: 7.40
Constituent: 95% PBS
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
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Functional Studies - Recombinant human TNF Receptor II protein (ab168884)
Recombinant human LTA protein (ab182708) at 5 µg/mL (100 µL/well) can bind ab168884 with a linear range of 2-62 ng/mL.
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SDS-PAGE - Recombinant human TNF Receptor II protein (ab168884)SDS PAGE of reduced ab168884 stained overnight with Coomassie Blue. The protein migrates as 35-45 kDa under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human TNF Receptor II protein (ab168884)Recombinant human TNF alpha protein (Active) (ab155699) at 5 µg/mL (100 µl/well) can bind ab168884 with a linear range of 5-39 ng/mL.
