Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (ab251276)
![Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (ab251276)](https://www.abcam.com/ps/products/251/ab251276/Images/ab251276-2-anti-pan-farnesylation-antibody-epr19409-bsa-and-azide-free-dot-blot.jpg "Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (ab251276)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19409] to Pan-Farnesylation - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free
See all Pan-Farnesylation primary antibodies -
Description
Rabbit monoclonal [EPR19409] to Pan-Farnesylation - BSA and Azide free -
Host species
Rabbit -
Specificity
The antibody was developed specifically for farnesylated LKB1. Refer to the literature (PMID: 24295069), it might also interact with other farnesylated proteins.
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Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251276 is the carrier-free version of ab199481. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251276 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19409 -
Isotype
IgG
Images
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Dot Blot - Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (ab251276)This data was developed using ab199481, the same antibody clone in a different buffer formulation.Dot blot analysis of Pan-Farnesylation labeled with ab199481 at 1/1000 dilution. Lane 1: LKB1 farnesylated peptide; Lane 2: LKB1 non-farnesylated peptide. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
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![Western blot - Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (ab251276)](https://www.abcam.com/ps/products/251/ab251276/Images/ab251276-399391-anti-pan-farnesylation-antibody-epr19409-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (ab251276)All lanes : Anti-Pan-Farnesylation antibody [EPR19409] (ab199481) at 1/1000 dilution
Lane 1 : HA-LKB1(WT) transfected 293T (Human epithelial cell line from embryonic kidney) lysate
Lane 2 : HA-LKB1(C433S) transfected 293T (Human epithelial cell line from embryonic kidney) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Exposure time: 2 secondsThis data was developed using ab199481, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by the collaborator (Dr. Sheng-Cai Lin, Xiamen University). Based on the sequence alignment, this product may recognize human, mouse and rat species. The ab was developed specifically for farnesylated LKB1. Refer to the literature (PMID: 24295069), it might also interact with other farnesylated proteins.
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![Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (ab251276)](https://www.abcam.com/ps/products/251/ab251276/Images/ab251276-3-benefits-of-recombinant-antibodies.png)
Anti-Pan-Farnesylation antibody [EPR19409] - BSA and Azide free (ab251276)