Recombinant human Semaphorin 3A protein (Fc Chimera Active) (ab233670)
Key features and details
- Expression system: CHO cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, HPLC
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Product name
Recombinant human Semaphorin 3A protein (Fc Chimera Active)
See all Semaphorin 3A proteins and peptides -
Biological activity
Determined by its ability to bind recombinant rat Neuropilin-1 Fc Chimera in a functional ELISA assay.
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Purity
>= 95 % SDS-PAGE.
>= 95% by HPLC analysis. -
Expression system
CHO cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
HHHHHHHHGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEER SRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECAN FIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGR GKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQH DSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIG QICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNF KDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQW VPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPM NNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPK ETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHR CDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRATRAQDIRNGDPLTH CSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEE RKEEIRVDDHIIRTDQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLE VIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQKVWYRDFMQLINHPNLN TMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFER APRSVGGPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEV TCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVL HQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELT KNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSK LTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
Predicted molecular weight
226 kDa -
Amino acids
25 to 771 -
Tags
His tag N-Terminus -
Additional sequence information
This product is the mature full length protein from aa 25 to 771. The signal peptide is not included. Fused with a 230-residue C-terminal Fc region linked by two glycines.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionLot numbers beginning with GR3223939 should be reconstituted in 1xPBS to 0.1-0.25 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 1 hour before use. All other lot numbers should be in 20mM Tris, pH 8.5 to a concentration of 0.1-0.25 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 2 hours before use. If you have any questions regarding the reconstitution of your product, please do not hesitate to contact our Scientific support team.
