Human MICA ELISA Kit (ab59569)
Key features and details
- Sensitivity: 123 pg/ml
- Range: 156 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MICA ELISA Kit
See all MICA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 6 2214.6pg/ml 50.3 2.3% B 6 1102.1pg/ml 39.5 3.6% C 6 641.6pg/ml 47.7 7.4% Inter-assay Sample n Mean SD CV% A 18 2416pg/ml 187.1 7.7% B 16 1200pg/ml 105.2 8.8% C 18 627pg/ml 50.9 8.1% -
Sample type
Cell culture supernatant, Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
= 123 pg/ml -
Range
156 pg/ml - 5000 pg/ml -
Assay time
3h 50m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s MICA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of MHC class 1 chain-related gene A glycoprotein, in cell culture supernatants and buffered solutions
A monoclonal antibody specific for MICA has been coated onto the wells of the microtiter strips provided. Samples, including standards of known MICA concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for MICA are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of MICA present in the samples.
This kit will recognize both endogenous and recombinant Human MICA.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 1X Standard Diluent Buffer 1 x 25ml 2 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-MICA (MHC Class I alpha) Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 23ml MICA Microplate (12 x 8 well strips) 1 unit 2 units MICA Standard (lyophilized) Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Relevance
The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system. -
Cellular localization
Plasma membrane -
Alternative names
- FLJ60820
- HLA class I antigen
- MGC111087
see all -
Database links
- Entrez Gene: 4276 Human
- Omim: 600169 Human
- SwissProt: Q29983 Human
- Unigene: 549053 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab59569.
