Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MICA + MICB antibody [EPR22071-91] (ab222098)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22071-91] to MICA + MICB
  • Suitable for: WB, IP
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-MICA + MICB antibody [EPR22071-91]

  • Description

    Rabbit monoclonal [EPR22071-91] to MICA + MICB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged MICA recombinant protein (aa24-307), His-tagged MICB (aa24-297; K562, HeLa, U266B1 and HUVEC whole cell lysates. IP: K562 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22071-91

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-MICA + MICB antibody [EPR22071-91] (ab222098)
    Immunoprecipitation - Anti-MICA + MICB antibody [EPR22071-91] (ab222098)

    MICA + MICB were immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab222098 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222098 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: K562 whole cell lysate 10 μg (Input).
    Lane 2: ab222098 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222098 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

  • Western blot - Anti-MICA + MICB antibody [EPR22071-91] (ab222098)
    Western blot - Anti-MICA + MICB antibody [EPR22071-91] (ab222098)
    All lanes : Anti-MICA + MICB antibody [EPR22071-91] (ab222098) at 1/1000 dilution

    Lane 1 : U266B1 (human multiple myeloma b lymphocyte) whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50-60 kDa why is the actual band size different from the predicted?


    Exposure time: 103 seconds


    Blocking and dilution buffer: 5% NFDM/TBST .

    Bands between 50-60kDa represent the glycosylated form.
    The molecular weight observed is consistent with what has been described in the literature (PMID: 10359807; 19147769; 21631944).

  • Western blot - Anti-MICA + MICB antibody [EPR22071-91] (ab222098)
    Western blot - Anti-MICA + MICB antibody [EPR22071-91] (ab222098)
    All lanes : Anti-MICA + MICB antibody [EPR22071-91] (ab222098) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : K562 whole cell lysate treated with PNGase F
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HeLa whole cell lysate treated with PNGase F

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa


    Exposure time: 136 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    Bands between 50-60kDa represent the glycosylated form. After PNGase F treatment, the de-glycosylated form appears around 40kDa.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10359807; 19147769; 21631944, 19568411).

  • Western blot - Anti-MICA + MICB antibody [EPR22071-91] (ab222098)
    Western blot - Anti-MICA + MICB antibody [EPR22071-91] (ab222098)
    All lanes : Anti-MICA + MICB antibody [EPR22071-91] (ab222098) at 1/1000 dilution

    Lane 1 : His-tagged MICA recombinant protein was expressed in E. coli (aa24-307)
    Lane 2 : His-tagged MICB recombinant protein was expressed in HEK-293 (aa24-297

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    The higher mass of MICB than that of MICA was due to glycosylation in the mammalian expression system.

  • Anti-MICA + MICB antibody [EPR22071-91] (ab222098)
    Anti-MICA + MICB antibody [EPR22071-91] (ab222098)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.