Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [16D10] to PCNA - BSA and Azide free
  • Suitable for: ICC, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PCNA antibody [16D10] - BSA and Azide free
    See all PCNA primary antibodies

  • Description

    Rat monoclonal [16D10] to PCNA - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: ICC, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: NIH/3T3, HeLa and C6 cells. IP: NIH/3T3 cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255842 is the carrier-free version of ab252848. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255842 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)
    Immunocytochemistry - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)

    Immunocytochemical analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast) cells labeling PCNA (green) using ab252848 at 7.08µg/ml followed by ab150157 Goat Anti-Rat  IgG H&L  (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in NIH/3T3 cell line. The nuclear counterdstain was DAPI (Blue). 

    -ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

    -ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

    This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848).

  • Immunoprecipitation - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)
    Immunoprecipitation - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)

    PCNA was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate using ab252848 at 1/30 dilution (2µg in 0.35µg lysates). Western blot was performed on the immunoprecipitate using ab252848 at 1/1000 dilution (0.35µg/ml) followed by anti-Rat IgG H&L (HRP) (ab205720) secondary at 1/5000 dilution. 

    Lane 1: NIH/3T3 whole cell lysate 10 µg.

    Lane 2: ab252848 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rat monoclonal IgG instead of ab252848 in NIH/3T3 whole cell lysate.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848).

  • Immunocytochemistry - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)
    Immunocytochemistry - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)

    Immunocytochemical analysis of 100% methanol-fixed C6 (rat glial tumor glial cell) cells labeling PCNA (green) using ab252848 at 7.08µg/ml followed by ab150157 Goat Anti-Rat  IgG H&L  (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in C6 cell line. The nuclear counterdstain was DAPI (Blue). 

    -ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

    -ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

    This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848)

  • Immunocytochemistry - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)
    Immunocytochemistry - Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)

    Immunocytochemical analysis of 100% methanol-fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PCNA (green) using ab252848 at 7.08µg/ml followed by ab150157 Goat Anti-Rat  IgG H&L  (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterdstain was DAPI (Blue). 

    -ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).

    -ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).

    This image was produced by the same antibody in a different buffer formulation containing PBS, BSA and sodium azide (ab252848)

  • Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)
    Anti-PCNA antibody [16D10] - BSA and Azide free (ab255842)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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