Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6069] to ARTS1/ERAP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARTS1/ERAP1 antibody [EPR6069]
    See all ARTS1/ERAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6069] to ARTS1/ERAP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human ARTS1/ERAP1 aa 900-1000 (C terminal).

  • Positive control

    • WB: K562, HUVEC, mouse heart, rat spleen and A431 cell lysates. IHC-P: Human uterus and human bladder carcinoma tissue. Flow Cyt: K562 cells.

  • General notes

     This product was previously labelled as ARTS1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified)

    Lane 1 : Mouse heart lysates
    Lane 2 : Rat spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/1000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP

    Predicted band size: 107 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

    Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
    Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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