Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6069] to ARTS1/ERAP1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-ARTS1/ERAP1 antibody [EPR6069]
See all ARTS1/ERAP1 primary antibodies
Description

Rabbit monoclonal [EPR6069] to ARTS1/ERAP1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human ARTS1/ERAP1 aa 900-1000 (C terminal).
Positive control
- WB: K562, HUVEC, mouse heart, rat spleen and A431 cell lysates. IHC-P: Human uterus and human bladder carcinoma tissue. Flow Cyt: K562 cells.
General notes
This product was previously labelled as ARTS1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6069
Isotype

IgG
Research areas

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 107 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified)

Lane 1 : Mouse heart lysates
Lane 2 : Rat spleen lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 107 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/1000 dilution (unpurified)

Lane 1 : K562 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP

Predicted band size: 107 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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