Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22081] to ILT-4 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ILT-4 antibody [EPR22081] - BSA and Azide free
    See all ILT-4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22081] to ILT-4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Human PBMC. Flow Cyt: Human PBMC.

  • General notes

    Ab243107 is the carrier-free version of ab224701. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243107 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22081

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)
    Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)

    Flow cytometric analysis of human primary peripheral blood mononuclear cell (PBMC) labeling ILT-4 with ab224701 at 1/500 (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat anti rabbit IgG (Dylight ® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with Alexa Fluor® 647-conjugated CD14 and rabbit IgG (Left) or ILT-4 (Right).

    Data shown were gated on viable cells.

    The CD14 co-staining result observed is consistent with what has been described in the literature (PMID:10879687).

    ILT-4 is expressed in monocytes and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224701).

  • Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)
    Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling ILT-4 with ab224701 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing co-staining with CD14 and cytoplasmic staining in PBMC cells. The nuclear counterstain is DAPI (blue). Counterstained with an Alexa Fluor® 647 anti-human CD14 antibody at a 1/100 dilution (red).

    The negative control is the secondary antibody only.

    ILT-4 is expressed in monocytes (PMID:10879687) and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224701).

  • Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)
    Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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