Anti-ILT-4 antibody [EPR22081] (ab224701)
![Anti-ILT-4 antibody [EPR22081] (ab224701)](https://www.abcam.com/ps/products/224/ab224701/Images/ab224701-332248-anti-ilt-4-antibody-epr22081-immunofluorescence.jpg "Anti-ILT-4 antibody [EPR22081] (ab224701)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22081] to ILT-4
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ILT-4 antibody [EPR22081]
See all ILT-4 primary antibodies -
Description
Rabbit monoclonal [EPR22081] to ILT-4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Human PBMC. Flow Cyt: Human PBMC.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22081 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] (ab224701)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling ILT-4 with ab224701 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing co-staining with CD14 and cytoplasmic staining in PBMC cells. The nuclear counterstain is DAPI (blue). Counterstained with an Alexa Fluor® 647 anti-human CD14 antibody at a 1/100 dilution (red).
The negative control is the secondary antibody only.
ILT-4 is expressed in monocytes (PMID:10879687) and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).
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![Flow Cytometry - Anti-ILT-4 antibody [EPR22081] (ab224701)](https://www.abcam.com/ps/products/224/ab224701/Images/ab224701-332249-anti-ilt-4-antibody-epr22081-flow-cytometry.jpg)
Flow Cytometry - Anti-ILT-4 antibody [EPR22081] (ab224701)Flow cytometric analysis of human primary peripheral blood mononuclear cell (PBMC) labeling ILT-4 with ab224701 at 1/500 (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat anti rabbit IgG (Dylight ® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with Alexa Fluor® 647-conjugated CD14 and rabbit IgG (Left) or ILT-4 (Right).
Data shown were gated on viable cells.
The CD14 co-staining result observed is consistent with what has been described in the literature (PMID:10879687).
ILT-4 is expressed in monocytes and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).
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![Anti-ILT-4 antibody [EPR22081] (ab224701)](https://www.abcam.com/ps/products/224/ab224701/Images/ab224701-3-benefits-of-recombinant-antibodies.png)
Anti-ILT-4 antibody [EPR22081] (ab224701)
