Anti-STK3/MST-2 antibody [3067C3a] (ab71960)
![Anti-STK3/MST-2 antibody [3067C3a] (ab71960)](https://www.abcam.com/ps/products/71/ab71960/Images/ab71960-395216-anti-stk3mst-2-antibody-3067c3a-western-blot-hela-lysates.jpg "Anti-STK3/MST-2 antibody [3067C3a] (ab71960)")
Key features and details
- Mouse monoclonal [3067C3a] to STK3/MST-2
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-STK3/MST-2 antibody [3067C3a]
See all STK3/MST-2 primary antibodies -
Description
Mouse monoclonal [3067C3a] to STK3/MST-2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment
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Immunogen
Recombinant fragment corresponding to Human STK3/MST-2.
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Positive control
- WB: HeLa, HAP1 and NIH3T3 cell lysates; Recombinant human STK3/MST-2.
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General notes
Previously labelled as STK3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab71960 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
3067C3a -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-STK3/MST-2 antibody [3067C3a] (ab71960)All lanes : Anti-STK3/MST-2 antibody [3067C3a] (ab71960) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : STK3 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDaLanes 1- 2: Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab71960 was shown to react with STK3/MST-2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265082 (knockout cell lysate ab257714) was used. Wild-type HeLa and STK3 knockout HeLa cell lysates were subjected to SDS-PAGE. ab71960 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-STK3/MST-2 antibody [3067C3a] (ab71960)](https://www.abcam.com/ps/products/71/ab71960/Images/ab71960-254535-ab71960WTKO.jpg)
Western blot - Anti-STK3/MST-2 antibody [3067C3a] (ab71960)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: STK3/MST-2 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: 293T cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab71960 was shown to recognize STK3/MST-2 when STK3/MST-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and STK3/MST-2 knockout samples were subjected to SDS-PAGE. ab71960 and ab181602 (loading control to STK3/MST-2) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-STK3/MST-2 antibody [3067C3a] (ab71960)](https://www.abcam.com/ps/products/71/ab71960/Images/ab71960.gif)
Western blot - Anti-STK3/MST-2 antibody [3067C3a] (ab71960)Anti-STK3/MST-2 antibody [3067C3a] (ab71960) + immunising recombinant protein
Predicted band size: 56 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
