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Cardiovascular Atherosclerosis Thrombosis Platelets

Anti-CD9 antibody (ab223052)

Price and availability

271 382 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-CD9 antibody (ab223052)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CD9
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CD9 antibody
    See all CD9 primary antibodies
  • Description

    Rabbit polyclonal to CD9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: African green monkey
  • Immunogen

    Recombinant fragment corresponding to Human CD9 aa 100-200.
    Database link: P21926

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse lung and kidney tissue lysates. HeLa, A549, HepG2, PC-3, U-87 MG and MCF7 whole cell lysates. IHC-P: Human placenta, breast cancer and colon cancer tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    >95% pure
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Developmental Biology
    • Reproduction
    • Fertilisation
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

Images

  • Western blot - Anti-CD9 antibody (ab223052)
    Western blot - Anti-CD9 antibody (ab223052)
    All lanes : Anti-CD9 antibody (ab223052) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 5 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)

    Immunohistochemistry of paraffin-embedded human breast cancer using ab223052 at dilution of 1:200.

  • Western blot - Anti-CD9 antibody (ab223052)
    Western blot - Anti-CD9 antibody (ab223052)
    All lanes : Anti-CD9 antibody (ab223052) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Mouse kidney lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)

    Paraffin embedded sections of human placenta tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)

    Paraffin embedded sections of human colon cancer tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-CD9 antibody [EM-04] (ab82390)

    Applications: WB

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    APC Anti-CD9 antibody [EM-04] (ab82392)

    Applications: Flow Cyt

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    APC/Cy7® Anti-CD9 antibody [MEM-61] (ab239306)

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