Anti-CD9 antibody (ab223052)
Key features and details
- Rabbit polyclonal to CD9
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD9 antibody
See all CD9 primary antibodies -
Description
Rabbit polyclonal to CD9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: African green monkey -
Immunogen
Recombinant fragment corresponding to Human CD9 aa 100-200.
Database link: P21926 -
Positive control
- WB: Mouse lung and kidney tissue lysates. HeLa, A549, HepG2, PC-3, U-87 MG and MCF7 whole cell lysates. IHC-P: Human placenta, breast cancer and colon cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
>95% pure -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CD9 antibody (ab223052) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 5 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using ab223052 at dilution of 1:200.
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All lanes : Anti-CD9 antibody (ab223052) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
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Paraffin embedded sections of human placenta tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.
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Paraffin embedded sections of human colon cancer tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.