Anti-SARS spike glycoprotein antibody - N-terminal (ab252691)
Key features and details
- Rabbit polyclonal to SARS spike glycoprotein - N-terminal
- Suitable for: WB, ELISA
- Isotype: IgG
Overview
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Product name
Anti-SARS spike glycoprotein antibody - N-terminal
See all SARS spike glycoprotein primary antibodies -
Description
Rabbit polyclonal to SARS spike glycoprotein - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Immunogen
Synthetic peptide corresponding to SARS spike glycoprotein (N terminal).
Database link: P59594 -
Positive control
- WB: SARS-Cov1 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SARS spike glycoprotein antibody - N-terminal (ab252691)All lanes : Anti-SARS spike glycoprotein antibody - N-terminal (ab252691) at 1 µg/ml
Lane 1 : SARS-Cov1 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lane 2 : SARS-Cov2 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lane 3 : MERS Spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 139 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Green - ab252691 observed at 200kDa.
ab252691 was shown to react with SARS spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab252691 overnight at 4°C at 1 µg/ml. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) secondary antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.