Recombinant human PCSK9 (mutated D374Y) protein (Active) (ab223014)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 92% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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ELISA - Recombinant human PCSK9 (mutated D374Y) protein (Active) (ab223014)
Immobilized Human LDL R, His Tag at 10 μg/mL (100 μL/well) can bind Recombinant human PCSK9 protein (ab223014) with a linear range of 0.004-0.063 μg/mL.
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Functional Studies - Recombinant human PCSK9 (mutated D374Y) protein (Active) (ab223014)Loaded Recombinant human LDL Receptor protein (Active) (ab220570) on Protein A Biosensor, can bind Recombinant human PCSK9 protein (ab223014) with an affinity constant of 0.329 nM as determined in BLI assay.
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SDS-PAGE - Recombinant human PCSK9 (mutated D374Y) protein (Active) (ab223014)Human PCSK9 (D374Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The protein migrates as 17 kDa and 66 kDa due to glycosylation and proteolytic digestion.
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Functional Studies - Recombinant human PCSK9 (mutated D374Y) protein (Active) (ab223014)FACS analysis shows that the effect of ab223014 inhibiting LDL uptake in HepG2 cells was neutralized by anti-human PCSK9 antibody. The concentration of PCSK9 used is 5 µg/ml. The EC50 for anti-human PCSK9 antibody is 6.816-12.67 µg/mL.
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Functional Studies - Recombinant human PCSK9 (mutated D374Y) protein (Active) (ab223014)FACS analysis shows that ab223014 inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 0.0689-0.3049 µg/mL.
