Recombinant hamster PCSK9 protein (Active) (ab221332)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
-
Functional Studies - Recombinant hamster PCSK9 protein (Active) (ab221332)
Immobilized Human LDL R (High Purity) Protein, Strep Tag at 5 μg/mL (100 μl/well) can bind ab221332 with a linear range of 6-50 ng/mL.
-
SDS-PAGE - Recombinant hamster PCSK9 protein (Active) (ab221332)SDS-PAGE analysis of reduced ab221332 stained overnight with Coomassie Blue.
The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.0 kDa respectively. The protein migrates as 18 kDa and 65 kDa under reducing (R) condition here due to glycosylation.
-
Functional Studies - Recombinant hamster PCSK9 protein (Active) (ab221332)Loaded Human LDL R, Fc Tag on Protein A Biosensor, can bind Hamster PCSK9, His Tag with an affinity constant of 4.09 nM as determined in BLI assay
