Recombinant rat PCSK9 protein (His tag) (ab201424)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 100 µg/ml.
Images
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ELISA - Recombinant Rat PCSK9 protein (ab201424)
Immobilized Recombinant human LDL Receptor protein (ab220582) can bind Recombinant Rat PCSK9 protein (ab201424) with a linear range of 8-63 ng/mL.
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SDS-PAGE - Recombinant Rat PCSK9 protein (ab201424)SDS-PAGE analysis of reduced ab201424 stained overnight with Coomassie Blue. The protein migrates as 16 kDa and 60-66 kDa under reducing condition due to glycosylation.
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Functional Studies - Recombinant rat PCSK9 protein (ab201424)Loaded Recombinant human LDL Receptor protein (ab220570) on Protein A Biosensor, can bind Recombinant rat PCSK9 protein (ab201424) with an affinity constant of 1.61 nM as determined in BLI assay.
