Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

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Anti-GSDMA antibody [EPR19858-104] (ab230768)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19858-104] to GSDMA
Suitable for: IHC-P, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-GSDMA antibody [EPR19858-104]
See all GSDMA primary antibodies
Description

Rabbit monoclonal [EPR19858-104] to GSDMA
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate; Hela cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate; mouse skin and stomach tissue lysate; human skin and stomach tissue lysate; rat skin tissue lysate. IP: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate. IHC-P: Human skin tissue; mouse stomach tissue; rat stomach tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19858-104
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] (ab230768)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of rat stomach (PMID: 23979942) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-GSDMA antibody [EPR19858-104] (ab230768)

All lanes : Anti-GSDMA antibody [EPR19858-104] (ab230768) at 1/1000 dilution

Lane 1 : Mouse skin tissue lysate
Lane 2 : Mouse stomach tissue lysate
Lane 3 : Human skin tissue lysate
Lane 4 : Human stomach tissue lysate
Lane 5 : Rat skin tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 49 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 32 seconds.

Lanes 2 & 3: 3 minutes.

Lanes 4 & 5: 102 seconds.

This image is produced using super sensitivity ECL substrate. We strongly suggest the customer to use higher sensitivity ECL substrate when developing the blot.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] (ab230768)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of mouse stomach (PMID: 23979942) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] (ab230768)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of human skin (PMID: 23979942) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-GSDMA antibody [EPR19858-104] (ab230768)

GSDMA was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab230768 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230768 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 μg (Input).
Lane 2: ab230768 IP in Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230768 in Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: less than 1 second.

The observed molecular mass is consistent with the literature (PMID:17350798).

The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
Western blot - Anti-GSDMA antibody [EPR19858-104] (ab230768)

All lanes : Anti-GSDMA antibody [EPR19858-104] (ab230768) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Hela cells stably transfected with mouse GSDMA2 expression vector, containing a DDDDK-tag, whole cell lysate
Lane 3 : Hela cells stably transfected with mouse GSDMA3 expression vector, containing a DDDDK-tag, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 49 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-GSDMA antibody [EPR19858-104] (ab230768)

All lanes : Anti-GSDMA antibody [EPR19858-104] (ab230768) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate
Lane 3 : Hela cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The observed molecular mass is consistent with the literature (PMID:17350798).
The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.
Anti-GSDMA antibody [EPR19858-104] (ab230768)

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