Denosumab ELISA Kit (Prolia®) (ab237649)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Denosumab ELISA Kit (Prolia®)
See all Denosumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3 ng/ml -
Range
3 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Denosumab ELISA Kit (Prolia®) (ab237649) has been developed for specific quantification of Denosumab concentration in human serum or plasma with high sensitivity and reproducibility. The density of color is proportional to the amount of Denosumab captured from the samples and can be quantified when compared with standard curve.
Denosumab (Prolia®) is a human monoclonal antibody that binds to RANKL, a transmembrane or soluble protein essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. Prolia prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors. Prevention of the RANKL/RANK interaction inhibits osteoclast formation, function, and survival, thereby decreasing bone resorption and increasing bone mass and strength in both cortical and trabecular bone.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Micro ELISA Plate 1 unit Denosumab Standard S1 1 x 1ml Denosumab Standard S2 1 x 1ml Denosumab Standard S3 1 x 1ml Denosumab Standard S4 1 x 1ml Denosumab Standard S5 1 x 1ml Denosumab Standard S6 1 x 1ml Denosumab Standard S7 1 x 1ml Denosumab Standard S8 1 x 1ml Assay Buffer 1 x 50ml HRP-conjugate Probe 1 x 12ml TMB substrate 1 x 12ml Stop Solution 1 x 12ml Wash buffer (20X) 1 x 50ml Plate sealers 2 units -
Relevance
Denosumab (Prolia®) is a human monoclonal antibody that binds to RANKL, a transmembrane or soluble protein essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. Prolia prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors. Prevention of the RANKL/RANK interaction inhibits osteoclast formation, function, and survival, thereby decreasing bone resorption and increasing bone mass and strength in both cortical and trabecular bone. -
Alternative names
- Prolia
Images
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Example of Denosumab standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.