Anti-Ku70 (phospho S5) antibody (ab61783)
Key features and details
- Rabbit polyclonal to Ku70 (phospho S5)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ku70 (phospho S5) antibody
See all Ku70 primary antibodies -
Description
Rabbit polyclonal to Ku70 (phospho S5) -
Host species
Rabbit -
Specificity
Detects endogenous levels of Ku70 only when phosphorylated at serine 5 (mouse homologue is Serine 6). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human Ku70 around the phosphorylation site of serine 5 (WESPYY)
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Positive control
- Extracts from HeLa cells (WB) Human lung carcinoma tissue (IHC)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 (phospho S5) antibody (ab61783)Immunohistochemistry analysis of paraffin embedded human lung carcinoma tissue using ab61783 at 1/50 dilution in the presence and abscence of immunizing peptide antibody.
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Western blot - Anti-Ku70 (phospho S5) antibody (ab61783)All lanes : Anti-Ku70 (phospho S5) antibody (ab61783) at 1/500 dilution
Lane 1 : extracts from HeLa cells with no immunizing peptide
Lane 2 : extracts from HeLa cells with immunizing peptide
Predicted band size: 70 kDa
Observed band size: 70 kDa
