Antibodies, Proteins, Kits and Reagents for Life Science

368 544 ₸ Product size

100 µl 368 544 ₸

Order now and get it on Tuesday March 09, 2021

Anti-JNK1 antibody [EPR140(2)] (ab110724)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR140(2)] to JNK1
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-JNK1 antibody [EPR140(2)]
See all JNK1 primary antibodies
Description

Rabbit monoclonal [EPR140(2)] to JNK1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK-293, K562, C6, RAW 264.7 and MCF7 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR140(2)
Isotype

IgG
Research areas

Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab110724 (unpurified) was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)

All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/2000 dilution

Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 46,54 kDa
why is the actual band size different from the predicted?
Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green).

Green - target observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

This western blot image is a comparison between ab110724 and a competitor's top cited mouse monoclonal antibody.
Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)

All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 48 kDa
Anti-JNK1 antibody [EPR140(2)] (ab110724)

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