Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)
![Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)](https://www.abcam.com/ps/products/185/ab185225/Images/ab185225-218734-rrrrrr.jpg "Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14412] to RRP7A - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-RRP7A antibody [EPR14412] - C-terminal
See all RRP7A primary antibodies -
Description
Rabbit monoclonal [EPR14412] to RRP7A - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF7, 293T, A431 and HeLa cell lysates; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14412 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)
Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling RRP7A with ab185225 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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![Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)](https://www.abcam.com/ps/products/185/ab185225/Images/ab185225-218732-1.jpg)
Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)All lanes : Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225) at 1/5000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 32 kDa
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![Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)](https://www.abcam.com/ps/products/185/ab185225/Images/ab185225-218733-2.jpg)
Western blot - Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225) at 1/1000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 32 kDa
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![Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)](https://www.abcam.com/ps/products/185/ab185225/Images/ab185225-4-benefits-of-recombinant-antibodies.png)
Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225)