Anti-CHD8 antibody (ab114126)
Key features and details
- Rabbit polyclonal to CHD8
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CHD8 antibody
See all CHD8 primary antibodies -
Description
Rabbit polyclonal to CHD8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 325-350 of Human CHD8 (NP_065971.2)
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Positive control
- HeLa whole cell lysate (ab150035)
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General notes
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99.91% Tris citrate/phosphate
pH: 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab114126 was affinity purified using an epitope specific to CHD8 immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CHD8 antibody (ab114126)All lanes : Anti-CHD8 antibody (ab114126) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 291 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD8 antibody (ab114126)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CHD8 with ab114126 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-CHD8 antibody (ab114126)ab114126 at 3ug/mg of lysate detecting CHD8 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
Detection: chemiluminescence. Exposure time: 30 seconds. -
Western blot - Anti-CHD8 antibody (ab114126)All lanes : Anti-CHD8 antibody (ab114126) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 291 kDa
Exposure time: 3 minutes
