Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD39 antibody [EPR3678(2)] (ab108248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3678(2)] to CD39
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-CD39 antibody [EPR3678(2)]
    See all CD39 primary antibodies

  • Description

    Rabbit monoclonal [EPR3678(2)] to CD39

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta, rat hippocampus, rat pancreas, mouse placenta and mouse urinary bladder tissue lysates; IM-9 and HUVEC cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CD39 antibody [EPR3678(2)] (ab108248)
    Western blot - Anti-CD39 antibody [EPR3678(2)] (ab108248)
    All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

    Lane 1 : Rat hippocampus
    Lane 2 : Rat pancreas

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 70,74 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration 5% NFDM/TBST.

  • Western blot - Anti-CD39 antibody [EPR3678(2)] (ab108248)
    Western blot - Anti-CD39 antibody [EPR3678(2)] (ab108248)
    All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

    Lane 1 : Mouse placenta
    Lane 2 : Mouse urinary bladder

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and diluting buffer and concentration 5% NFDM/TBST.

    The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.

  • Western blot - Anti-CD39 antibody [EPR3678(2)] (ab108248)
    Western blot - Anti-CD39 antibody [EPR3678(2)] (ab108248)
    Lane 1 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/100 dilution (purified)
    Lane 2 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

    All lanes : HUVEC whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 70,74 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer and concentration 5% NFDM/TBST.

    Ref: PMID: 18693757

  • Western blot - Anti-CD39 antibody [EPR3678(2)] (ab108248)
    Western blot - Anti-CD39 antibody [EPR3678(2)] (ab108248)
    All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (unpurified)

    Lane 1 : Human placenta lysate
    Lane 2 : IM-9 cell lysate

    Predicted band size: 58 kDa

  • Anti-CD39 antibody [EPR3678(2)] (ab108248)
    Anti-CD39 antibody [EPR3678(2)] (ab108248)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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