Anti-CD28 antibody (ab113358)
Key features and details
- Goat polyclonal to CD28
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD28 antibody
See all CD28 primary antibodies -
Description
Goat polyclonal to CD28 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
SQQLQVYSKTGFNCD
, corresponding to internal sequence amino acids 73-87 of Human CD28 (NP_006130.1). -
Positive control
- Human lymph node and tonsil lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab113358 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)
ab113358 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)ab113358 negative control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
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Western blot - Anti-CD28 antibody (ab113358)Anti-CD28 antibody (ab113358) at 0.3 µg/ml + Human tonsil lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Note: The observed molecular weight corresponds to the glycosylated form.
