Anti-CD28 antibody [EPR22311-17] - BSA and Azide free (ab242154)
![Anti-CD28 antibody [EPR22311-17] - BSA and Azide free (ab242154)](https://www.abcam.com/ps/products/242/ab242154/Images/ab242154-330839-anti-cd28-antibody-epr22311-17-immunoprecipitation.jpg "Anti-CD28 antibody [EPR22311-17] - BSA and Azide free (ab242154)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22311-17] to CD28 - BSA and Azide free
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse
Overview
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Product name
Anti-CD28 antibody [EPR22311-17] - BSA and Azide free
See all CD28 primary antibodies -
Description
Rabbit monoclonal [EPR22311-17] to CD28 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse spleen tissue. IP: EL4.IL-2 whole cell lysate.
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General notes
Ab242154 is the carrier-free version of ab242124. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab242154 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22311-17 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CD28 antibody [EPR22311-17] - BSA and Azide free (ab242154)
CD28 was immunoprecipitated from 0.35 mg EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate with ab242124 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab242124 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: EL4.IL-2 whole cell lysate 10 µg (input).
Lane 2: ab242124 IP in EL4.IL-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242124 in EL4.IL-2 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242124).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody [EPR22311-17] - BSA and Azide free (ab242154)](https://www.abcam.com/ps/products/242/ab242154/Images/ab242154-330838-anti-cd28-antibody-epr22311-17-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody [EPR22311-17] - BSA and Azide free (ab242154)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue stained for CD28 using ab242124 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in periarterial lymphatic sheath of mouse spleen (PMID: 7489738). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
The section was incubated with ab242124 for 30 mins at 37℃. Immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (a242124).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-CD28 antibody [EPR22311-17] - BSA and Azide free (ab242154)](https://www.abcam.com/ps/products/242/ab242154/Images/ab242154-3-benefits-of-recombinant-antibodies.png)
Anti-CD28 antibody [EPR22311-17] - BSA and Azide free (ab242154)
