Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-CD39 antibody [EPR20627] (ab223842)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20627] to CD39
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-CD39 antibody [EPR20627]
See all CD39 primary antibodies
Description

Rabbit monoclonal [EPR20627] to CD39
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal heart, fetal spleen, placenta and tonsil lysates; Mouse placenta, brain, heart and kidney lysates. IHC-P: Human colon carcinoma, spleen and liver tissues; Mouse spleen tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20627
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID: 25403716). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CD39 antibody [EPR20627] (ab223842)

Lanes 1-2 : Anti-CD39 antibody [EPR20627] (ab223842) at 1/1000 dilution
Lanes 3-5 : Anti-CD39 antibody [EPR20627] (ab223842) at 1/10000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human placenta lysate
Lane 3 : Human fetal spleen lysate
Lane 4 : Human tonsil lysate
Lane 5 : Mouse placenta lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 57 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

Exposure time : Lane 1: 30 seconds; Lane 2: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 18036594; PMID: 10636909).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human spleen (PMID: 22684996; PMID: 27532024). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse spleen (PMID: 22684996; PMID: 27532024). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human liver (PMID: 18567823; PMID: 19308674). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CD39 antibody [EPR20627] (ab223842)

All lanes : Anti-CD39 antibody [EPR20627] (ab223842) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 57 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Exposure times: Lane 1: 30 seconds; Lane 2: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 18036594; PMID: 10636909).
Anti-CD39 antibody [EPR20627] (ab223842)

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