Anti-HER2 ELISA Kit (ab237645)
Key features and details
- Sensitivity: 11 ng/ml
- Range: 11 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Anti-HER2 ELISA Kit
See all Trastuzumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
11 ng/ml -
Range
11 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Anti-HER2 ELISA Kit (ab237645) is a highly specific and sensitive kit designed for the in vitro determination of anti-HER2 in biological matrices such as human serum and plasma. The density of color is proportional to the amount of anti-HER2 captured from the samples and can be quantified when compared with standard curve.
Anti-HER2 (Trastuzumab, Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Anti-HER2 has anti-tumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. This antibody was approved in 1998 for clinical use for HER2 overexpressing metastatic breast cancer. In HER2 overexpressing cells, anti-HER2 markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Anti-HER2 Standard S1 1 x 300µl Anti-HER2 Standard S2 1 x 300µl Anti-HER2 Standard S3 1 x 300µl Anti-HER2 Standard S4 1 x 300µl Anti-HER2 Standard S5 1 x 300µl Anti-HER2 Standard S6 1 x 300µl Anti-HER2 Standard S7 1 x 300µl Assay Buffer 2 x 50ml HRP-conjugate Probe 1 x 12ml Micro ELISA Plate 1 unit Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Relevance
Trastuzumab (Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Trastuzumab has antitumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. This antibody was approved in 1998 for clinical use for HER2 overexpressing metastatic breast cancer. In HER2 overexpressing cells, trastuzumab markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes. B -
Alternative names
- Herceptin
- Herclon
- human epidermal growth factor receptor 2
Images
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Example of Trastuzumab standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.
