Antibodies, Proteins, Kits and Reagents for Life Science

Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR677(2)] to EpCAM - BSA and Azide free
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free
See all EpCAM primary antibodies
Description

Rabbit monoclonal [EPR677(2)] to EpCAM - BSA and Azide free
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HCT116, A431 and HT29 cell lysates. IHC-P: Human colon adenocarcinoma, colon, endometrial adenocarcinoma and stomach adenocarcinoma tissues.
General notes
Ab219364 is the carrier-free version of ab124825. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab219364 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR677(2)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human colon tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124825).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human endometrial adenocarcinoma tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124825).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

ab124825 showing positive staining in Urinary bladder transitional carcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124825).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

ab124825 showing negative staining in Normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124825).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

ab124825 showing negative staining in Glioma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124825).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

ab124825 showing negative staining in Skeletal muscle tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124825).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

ab124825 showing negative staining in Normal tonsil tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124825).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human stomach adenocarcinoma tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124825).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

This IHC data was generated using the same anti-EpCAM antibody clone, EPR677(2), in a different buffer formulation (cat# ab124825). Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

This IHC data was generated using the same anti-EpCAM antibody clone, EPR677(2), in a different buffer formulation (cat# ab124825). Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

ab124825 showing positive staining in Normal kidney tissue.
Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free (ab219364)

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