Rat ST2 ELISA Kit (IL1RL1) (ab255716)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.36 pg/ml
- Range: 70.31 pg/ml - 4500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat ST2 ELISA Kit (IL1RL1)
See all ST2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3% Inter-assay Sample n Mean SD CV% Serum 3 7% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.36 pg/ml -
Range
70.31 pg/ml - 4500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 82 81% - 83% Hep Plasma 88 83% - 94% EDTA Plasma 90 81% - 102% Cit plasma 90 81% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat
Does not react with: Cow -
Product overview
Rat ST2 ELISA Kit (IL1RL1) (ab255716) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ST2 (IL1RL1) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat ST2 (IL1RL1) with 5.36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ST2, also known as or Interleukin 1 receptor-like 1 (IL-1RL1), is a protein encoded by the IL1RL1 gene, a member of the interleukin 1 receptor family, specifically the receptor for interleukin-33. IL-33 binding to ST2 induces MYD88, IRAK1, IRAK4, and TRAF6 recruitment and subsequent phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Studies of the homologous gene in mouse reported protein induction by pro-inflammatory stimuli with involvement in the function of helper T cells. ST2 is necessary for endotoxin tolerance by inhibiting TLR responses inducing the Th2 response. Rat ST2 shares 80.1%, 63.3%, 52.9%, and 61.6% sequence homology with mouse, human, bovine, and monkey, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat ST2 (IL1RL1) Capture Antibody 1 x 600µl 10X Rat ST2 (IL1RL1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat ST2 (IL1RL1) Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. -
Tissue specificity
Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- DER-4
- DER4
- FIT 1
see all -
Database links
- Entrez Gene: 25556 Rat
- SwissProt: Q62611 Rat
- Unigene: 10072 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat ST2 standard curve in Sample Diluent NS.The ST2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ST2 in rat serum and plasma samples.The concentrations of ST2 were measured in duplicates, interpolated from the ST2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 50% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 656.39 pg/mL in serum, 767.97 pg/mL in plasma (citrate), 534.46 pg/mL in plasma (EDTA), and 531.74 pg/mL in plasma (heparin).
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Sandwich ELISA - Rat ST2 ELISA Kit (IL1RL1) (ab255716)To learn more about the advantages of recombinant antibodies see here.
