Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)
![Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-454311-anti-glutamate-receptor-4antibody-epr2428369-western-blot-lysate1.jpg "Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24283-69] to Glutamate receptor 4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Glutamate receptor 4 antibody [EPR24283-69]
See all Glutamate receptor 4 primary antibodies -
Description
Rabbit monoclonal [EPR24283-69] to Glutamate receptor 4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat cerebellum and brain; Mouse cerebellum and brain; Human cerebellum tissue lysates. IHC-P: Mouse cerebrum; Rat cerebrum; Human cerebrum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24283-69 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)All lanes : Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate at 20 µg
Lane 2 : Human liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)
Negative control: human liver (PMID:1372042)
Exposure time: 3.25 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-3-anti-glutamate-receptor-4antibody-epr2428369-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-454310-anti-glutamate-receptor-4antibody-epr2428369-western-blot-lysate.jpg)
Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)All lanes : Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution
Lane 1 : Rat cerebellum tissue lysate at 20 µg
Lane 2 : Rat brain tissue lysate at 20 µg
Lane 3 : Rat liver tissue lysate at 40 µg
Lane 4 : Mouse cerebellum tissue lysate at 20 µg
Lane 5 : Mouse brain tissue lysate at 20 µg
Lane 6 : Mouse liver tissue lysate at 40 µg
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Lanes 2-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)
Negative control: liver (PMID:1372042)
Exposure time: 7.75 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-1-anti-glutamate-receptor-4antibody-epr2428369-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum. (PMID: 20662939) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-2-anti-glutamate-receptor-4antibody-epr2428369-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-4-anti-glutamate-receptor-4antibody-epr2428369-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse liver. (PMID: 1372042) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins