Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)
![Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)](https://www.abcam.com/ps/products/239/ab239861/Images/ab239861-414400-AntiCD32B-phospho-Y292-antibody.jpg "Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP926Y] to CD32B (phospho Y292) - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free
See all CD32B primary antibodies -
Description
Rabbit monoclonal [EP926Y] to CD32B (phospho Y292) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Raji whole cell lysate WB: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
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General notes
ab239861 is the carrier-free version of ab68423 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab239861 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP926Y -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)
ab68423 at 1/20 dilution immunoprecipitating CD32B in Raji (Human Burkitt's lymphoma) whole cell lysates.
Lane 1 (input): Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
Lane 2 (+): Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
For western blotting, ab68423 at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.Expsoure time: 1 second
Observed MW: 40-44 KDa
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![Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)](https://www.abcam.com/ps/products/239/ab239861/Images/ab239861-414398-AntiCD32B-phospho-Y292-antibody.jpg)
Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)All lanes : Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40,44 kDaExposure time: 5 seconds
Observed MW: 40-44 KDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
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![Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)](https://www.abcam.com/ps/products/239/ab239861/Images/ab239861-3-benefits-of-recombinant-antibodies.png)
Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)
