Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP926Y] to CD32B (phospho Y292) - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free
    See all CD32B primary antibodies

  • Description

    Rabbit monoclonal [EP926Y] to CD32B (phospho Y292) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Raji whole cell lysate WB: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate

  • General notes

    ab239861 is the carrier-free version of ab68423 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239861 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)
    Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)

    ab68423 at 1/20 dilution immunoprecipitating CD32B  in Raji (Human Burkitt's lymphoma) whole cell lysates. 
    Lane 1 (input): Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
    Lane 2 (+): Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
    For western blotting, ab68423  at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

    Expsoure time: 1 second

    Observed MW: 40-44 KDa

  • Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)
    Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)
    All lanes : Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution

    Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
    Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40,44 kDa



    Exposure time: 5 seconds

    Observed MW: 40-44 KDa

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

  • Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)
    Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free (ab239861)

© 2021 Astana Biomed Group LLP. All rights reserved.