Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
![Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)](https://www.abcam.com/ps/products/68/ab68423/Images/ab68423-414399-AntiCD32B-phospho-Y292-antibody.jpg "Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP926Y] to CD32B (phospho Y292)
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-CD32B (phospho Y292) antibody [EP926Y]
See all CD32B primary antibodies -
Description
Rabbit monoclonal [EP926Y] to CD32B (phospho Y292) -
Host species
Rabbit -
Specificity
Detects CD32b only when phosphorylated on Tyrosine 292. -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
corresponding to Human CD32B.
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Positive control
- IP: Raji whole cell lysate WB: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP926Y -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
ab68423 at 1/20 dilution immunoprecipitating CD32B in Raji (Human Burkitt's lymphoma) whole cell lysates.
Lane 1 (input): Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
Lane 2 (+): Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
For western blotting, ab68423 at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.Expsoure time: 1 second
Observed MW: 40-44 KDa
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![Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)](https://www.abcam.com/ps/products/68/ab68423/Images/ab68423-414397-AntiCD32B-phospho-Y292-antibody.jpg)
Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)All lanes : Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40,44 kDaExposure time: 5 seconds
Observed MW: 40-44 KDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
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![Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)](https://www.abcam.com/ps/products/68/ab68423/Images/ab68423-4-benefits-of-recombinant-antibodies.png)
Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
