Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD116 antibody [EPR24554-113] (ab280357)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24554-113] to CD116
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD116 antibody [EPR24554-113]
    See all CD116 primary antibodies

  • Description

    Rabbit monoclonal [EPR24554-113] to CD116

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW264.7 and J774A.1 whole cell lysate. Mouse lung and spleen lysate. IHC-P: Mouse lung and spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD116 antibody [EPR24554-113] (ab280357)
    Western blot - Anti-CD116 antibody [EPR24554-113] (ab280357)
    All lanes : Anti-CD116 antibody [EPR24554-113] (ab280357) at 1/1000 dilution

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : J774A.1 (mouse reticum cell sarcoma monocyte macrophage), whole cell lysate
    Lane 3 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 60-70 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Expression of GMRa is restrcted to cells of the myeloid, but not lymphoid (PMID 17694571).

    No expression of GMRa is observed in mouse B cell lymphoma B lymphocyte WEHI-231.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18955570).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR24554-113] (ab280357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR24554-113] (ab280357)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD116 with ab280357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse spleen. The section was incubated with ab280357 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-CD116 antibody [EPR24554-113] (ab280357)
    Western blot - Anti-CD116 antibody [EPR24554-113] (ab280357)
    All lanes : Anti-CD116 antibody [EPR24554-113] (ab280357) at 1/1000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 60-70 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: Lane 1: 80 seconds Lane 2: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR24554-113] (ab280357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR24554-113] (ab280357)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD116 with ab280357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sporadic positive staining in mouse lung. The section was incubated with ab280357 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-CD116 antibody [EPR24554-113] (ab280357)
    Anti-CD116 antibody [EPR24554-113] (ab280357)

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