Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S106)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Estrogen Receptor alpha (phospho S106) antibody
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor alpha (phospho S106) -
Host species
Rabbit -
Specificity
ab131111 detects Estrogen Receptor alpha only when phosphorylated at serine 106. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic phosphopeptide conjugated to KLH, surrounding phosphorylation site of serine 106 (S-P-S(p)-P-L) derived from Human Estrogen Receptor alpha (NP_000116.2).
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Positive control
- MCF cells; MCF7 cell extracts treated with EGF; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131111 was affinity-purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling Estrogen Receptor alpha (phospho S106) with ab131111 at 1/50 dilution. The image on the right is treated blocking peptide.
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Western blot - Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111)All lanes : Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111) at 1/500 dilution
Lane 1 : MCF7 cell extracts, untreated
Lane 2 : MCF7 cell extracts, treated with EGF
Predicted band size: 66 kDa
Incubate membrane with diluted antibody in 5% non-fat milk, 1 x TBS, 0.1% Tween20 at 4°C overnight with shaking. -
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S106) antibody (ab131111)Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF cells labelling Estrogen Receptor alpha (phospho S106) with ab131111 at 1/100 dilution.
