Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)
Key features and details
- Rabbit monoclonal [EP6243] to CD32B + CD32A + CD32C
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD32B + CD32A + CD32C antibody [EP6243] -
Description
Rabbit monoclonal [EP6243] to CD32B + CD32A + CD32C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human CD32A aa 1-100 (extracellular). The exact sequence is proprietary.
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Positive control
- Daudi, U937, Raji, and Human pancreas lysates; Human spleen tissue. ICC/IF: Daudi cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP6243 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD32B + CD32A + CD32C with ab131051 antibody at 1/100 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence analysis of Daudi (human Burkitt's lymphoma lymphoblast) cells labeling CD32B + CD32A + CD32C with purified ab131051 at 1/500 dilution (4.6 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.
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All lanes : Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Human pancreas lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Actual band size : 37-43 kDa