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Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [143F] to XBP1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-XBP1 antibody [143F] - BSA and Azide free
    See all XBP1 primary antibodies

  • Description

    Mouse monoclonal [143F] to XBP1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: ChIP,Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Huh7+ thapsigargin (300nM 18h) lysate. IHC-P: Human non-Hodgkin's lymphoma and tonsil tissue. ICC/IF: Huh7 cells treated with thapsigargin (300 nM) for 18 hours.

  • General notes

    ab252780 is a PBS only version of ab241571.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)
    Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling XBP1 with ab241571 at 11.66 µg/ml, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Confocal image showing increased signal staining in Huh7 cells treated with thapsigargin (300 nM) for 18 hours. Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab241571).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling XBP1 with ab241571 at 1.166 µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on the human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab241571).

  • Western blot - Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)
    Western blot - Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)
    All lanes : Anti-XBP1 antibody [143F] (ab241571) at 1/1000 dilution

    Lane 1 : Huh7+ thapsigargin (300nM 18h) (-) lysate
    Lane 2 : Huh7+ thapsigargin (300nM 18h) (+) lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab241571).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)

    Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma tissue labeling XBP1 with ab241571 at 1.166 µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on the non-Hodgkin's lymphoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab241571).

  • Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)
    Anti-XBP1 antibody [143F] - BSA and Azide free (ab252780)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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