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Epigenetics and Nuclear Signaling Transcription Other factors

Human CNOT7 knockout HeLa cell lysate (ab258370)

Price and availability

331 689 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Human CNOT7 knockout HeLa cell lysate (ab258370)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human CNOT7 knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.
  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261219 - Human CNOT7 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Function

    Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression.
  • Sequence similarities

    Belongs to the CAF1 family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9UIV1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • BTG1 binding factor 1
    • BTG1-binding factor 1
    • CAF 1
    • CAF-1
    • CAF1
    • Carbon catabolite repressor protein (CCR4) associative factor 1
    • CCR4 associated factor 1
    • CCR4 NOT transcription complex subunit 7
    • CCR4-associated factor 1
    • CCR4-NOT transcription complex subunit 7
    • CNOT 7
    • Cnot7
    • CNOT7_HUMAN
    • hCAF 1
    • hCAF1
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261219 - Human CNOT7 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Images

  • Western blot - Human CNOT7 knockout HeLa cell lysate (ab258370)
    Western blot - Human CNOT7 knockout HeLa cell lysate (ab258370)

    Lane 1:Wild-type HeLa cell lysate (20 ug)
    Lane 2:CNOT7 knockout HeLa cell lysate (20 ug)
    Lane 3:MOLT-4 cell lysate (20 ug)

    ab195587 was shown to specifically react with CNOT7 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265811 (knockout cell lysate ab258370) was used. Wild-type and CNOT7 knockout samples were subjected to SDS-PAGE. ab195587 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human CNOT7 knockout HeLa cell lysate (ab258370)
    Sanger Sequencing - Human CNOT7 knockout HeLa cell lysate (ab258370)
    Homozygous: 1 bp deletion in exon2

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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