Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)
![Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)](https://www.abcam.com/ps/products/233/ab233045/Images/ab233045-1-ICCAP46556671.jpg "Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [9C8] to DDIT3
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG2b
Overview
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Product name
Alexa Fluor® 647 Anti-DDIT3 antibody [9C8]
See all DDIT3 primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [9C8] to DDIT3 -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Fusion protein within DDIT3. The exact sequence is proprietary. A bacterially expressed, mouse DDIT3 fusion protein.
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Epitope
ab11419 has been shown to recognize an epitope in the N-terminal region of DDIT3. -
Positive control
- ICC/IF: Tunicamycin (1.5uM, 6hr) treated HeLa cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
9C8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)
ab233045 staining DDIT3 in HeLa cells +/- Tunicamycin (1.5uM, 6hr). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab233045 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa treated with Tunicamycin (1.5uM, 6hr) cells fixed with 100% methanol (5 min).
