Anti-HEXIM1 antibody (ab58573)
Key features and details
- Goat polyclonal to HEXIM1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HEXIM1 antibody
See all HEXIM1 primary antibodies -
Description
Goat polyclonal to HEXIM1 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human HEXIM1 aa 346-359 (C terminal).
Sequence:C-HRQQERAPLSKFGD
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Positive control
- WB: Human and mouse heart tissue lysates. ICC/IF: HeLa and A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HEXIM1 antibody (ab58573)All lanes : Anti-HEXIM1 antibody (ab58573) at 0.1 µg/ml
Lane 1 : Human heart tissue lysate in RIPA buffer
Lane 2 : Mouse heart tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDaIncubated with the primary antibody for 1 hour.
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Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody (ab58573)Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation for 1hr with ab58573 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody (ab58573)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation for 1hr with ab58573 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
