Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)
![Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)](https://www.abcam.com/ps/products/251/ab251383/Images/ab251383-367578-anti-crispr-cas9-antibody-epr19620-immunocytochemistry-immunofluorescence.jpg "Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19633] to CRISPR-Cas9 - BSA and Azide free
- Suitable for: Flow Cyt, WB, IP, ICC/IF
Overview
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Product name
Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free
See all CRISPR-Cas9 primary antibodies -
Description
Rabbit monoclonal [EPR19633] to CRISPR-Cas9 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Streptococcus thermophilus -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251383 is the carrier-free version of ab202657. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251383 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19633 -
Isotype
IgG
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag or Empty vector, labeling CRISPR-Cas9 with ab202657 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150079) secondary antibody at 1/1000 dilution.
Confocal image showing Positive staining on HeLa cells transfected with CRISPR-Cas9(G3ECR1, Streptococcus thermophilus) with Myc-His tag.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab202657 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202657).
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![Flow Cytometry - Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)](https://www.abcam.com/ps/products/251/ab251383/Images/ab251383-367579-anti-crispr-cas9-antibody-epr19620-flow-cytometry.jpg)
Flow Cytometry - Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag labeling CRISPR-Cas9 with ab202657 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730)(black). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202657).
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![Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)](https://www.abcam.com/ps/products/251/ab251383/Images/ab251383-367580-anti-crispr-cas9-antibody-epr19620-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)CRISPR-Cas9 was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag with ab202657 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab202657 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag 10µg (Input).
Lane 2: ab202657 IP in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab202657 in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202657).
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![Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)](https://www.abcam.com/ps/products/251/ab251383/Images/ab251383-4-benefits-of-recombinant-antibodies.png)
Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (ab251383)
