Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
![Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)](https://www.abcam.com/ps/products/3/ab3980/Images/ab3980-8451-anti-alpha-1-fetoprotein-antibody-afp-01-immunofluorescence.jpg "Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)")
Key features and details
- Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-alpha 1 Fetoprotein antibody [AFP-01]
See all alpha 1 Fetoprotein primary antibodies -
Description
Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein -
Host species
Mouse -
Specificity
The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.
-
Positive control
- Hep G2 human hepatocellular carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified by precipitation and chromatography -
Clonality
Monoclonal -
Clone number
AFP-01 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
