Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] (ab241324)
![Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] (ab241324)](https://www.abcam.com/ps/products/241/ab241324/Images/ab241324-1-241324ELISA.jpg "Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] (ab241324)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CPT-R19-48-7] to alpha 1 Fetoprotein
- Suitable for: ELISA
- Reacts with: Human
Overview
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Product name
Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7]
See all alpha 1 Fetoprotein primary antibodies -
Description
Rabbit monoclonal [CPT-R19-48-7] to alpha 1 FetoproteinThis product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ELISA: CPTAC-23a peptide.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.59% Sodium citrate, 0.03% EDTA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CPT-R19-48-7 -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] (ab241324)
100 ng CPTAC-23a (2000 ng/mL, 50 μL per well) was coated onto 96-wells.
Serial dilutions (0, 0.01, 0.02, 0.05, 0.15, 0.44, 1.33, 4.00 μg/mL) of ab241324 (50 μL) were incubated with CPTAC-23a in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50 μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development.
OD was read at 405nm within 5 minutes.
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![Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] (ab241324)](https://www.abcam.com/ps/products/241/ab241324/Images/ab241324-2-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] (ab241324)
