Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MTF2 antibody [EPR23536-87] - ChIP Grade – BSA and Azide free (ab275967)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23536-87] to MTF2 - ChIP Grade – BSA and Azide free
  • Suitable for: WB, IP, ChIP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MTF2 antibody [EPR23536-87] - ChIP Grade – BSA and Azide free
    See all MTF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23536-87] to MTF2 - ChIP Grade – BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ChIPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse skeletal muscle and testis tissue lysates; Rat testis tissue lysate; Human testis tissue lysate; HeLa, HEK-293T, ES-D3, C6 and NIH/3T3 whole cell lysates. IP: ES-D3 whole cell lysate; HeLa whole cell lysate. ChIP: Chromatin prepared from ED-D3 cells.

  • General notes

    ab275967 is the carrier-free version of ab254336. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275967 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • ChIP - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    ChIP - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)

    This data was developed using ab254336, the same antibody clone in a different buffer formulation. 

    Chromatin was prepared from ES-D3 cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab254336 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers are from paper PMID:20123894

  • Western blot - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    Western blot - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    Anti-MTF2 antibody [EPR23536-87] - ChIP Grade (ab254336) at 1/1000 dilution + Human testis tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa
    Observed band size: 47,56,67 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab254336, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    Immunoprecipitation - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)

    This data was developed using ab254336, the same antibody clone in a different buffer formulation.

    MTF2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab254336 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254336 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab254336 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254336 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds.

    67, 56, 47, 29-kDa bands correspond to MTF2 isoforms.This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    Western blot - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    All lanes : Anti-MTF2 antibody [EPR23536-87] - ChIP Grade (ab254336) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle tissue lysate
    Lane 2 : Mouse testis tissue lysate
    Lane 3 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 67 kDa
    Observed band size: 47,56,67 kDa why is the actual band size different from the predicted?



    This data was developed using ab254336, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 92 seconds.

  • Immunoprecipitation - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    Immunoprecipitation - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)

    This data was developed using ab254336, the same antibody clone in a different buffer formulation.

    MTF2 was immunoprecipitated from 0.35 mg ES-D3 (mouse embryonic multipotent stem Cell) whole cell lysate with ab254336 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254336 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: ES-D3 (mouse embryonic multipotent stem Cell) whole cell lysate 10 ug

    Lane 2: ab254336 IP in ES-D3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254336 in ES-D3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    67, 56, 47, 29-kDa bands correspond to MTF2 isoforms. Bands between 30-37 kDa is caused by degradation as demonstrated by WB data.

  • Western blot - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    Western blot - Anti-MTF2 antibody [EPR23536-87] - BSA and Azide free (ab275967)
    All lanes : Anti-MTF2 antibody [EPR23536-87] - ChIP Grade (ab254336) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lanes 3 & 6 : ES-D3 (mouse embryonic mtipotent stem Cell), whole cell lysate 20
    Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 67 kDa
    Observed band size: 47,56,67 kDa why is the actual band size different from the predicted?



    This data was developed using ab254336, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28869966).

    Lysate loaded onto lane 6 was made freshly and used in WB immediately to minimize protein degradation. 

    67, 56, 47, 29-kDa bands correspond to MTF2 isoforms. Bands between 30-37 kDa in lane3 is caused by degradation.

     Exposure time: Lanes 1-2: 26 seconds; Lane 3: 10 seconds; Lanes 4-5: 125 seconds; Lane 6: 3 minutes.

  • Anti-MTF2 antibody [EPR23536-87] - ChIP Grade – BSA and Azide free (ab275967)
    Anti-MTF2 antibody [EPR23536-87] - ChIP Grade – BSA and Azide free (ab275967)

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