Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6177(2)] to ALKBH2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free
    See all ALKBH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6177(2)] to ALKBH2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab243917 is the carrier-free version of ab154859. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243917 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
    Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
    Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab154859, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
    Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
    All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified)

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 29 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    This data was developed using ab154859, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
    Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
    All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution

    Lanes 1 & 3 : Human fetal brain tissue lysate
    Lane 2 : Human testis tissue lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 29 kDa



    This data was developed using ab154859, the same antibody clone in a different buffer formulation.

  • Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
    Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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