Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
![Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)](https://www.abcam.com/ps/products/243/ab243917/Images/ab243917-408075-anti-alkbh2-antibody-epr6177-2-bsa-and-azide-free-western-blot.jpg "Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6177(2)] to ALKBH2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free
See all ALKBH2 primary antibodies -
Description
Rabbit monoclonal [EPR6177(2)] to ALKBH2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab243917 is the carrier-free version of ab154859. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab243917 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6177(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?This data was developed using ab154859, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)](https://www.abcam.com/ps/products/243/ab243917/Images/ab243917-405614-anti-alkbh2-antibody-epr6177-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified)
Lane 1 : Human fetal brain tissue lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?This data was developed using ab154859, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)](https://www.abcam.com/ps/products/243/ab243917/Images/ab243917-402404-anti-alkbh2-antibody-epr6177-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution
Lanes 1 & 3 : Human fetal brain tissue lysate
Lane 2 : Human testis tissue lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDaThis data was developed using ab154859, the same antibody clone in a different buffer formulation.
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![Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)](https://www.abcam.com/ps/products/243/ab243917/Images/ab243917-6-benefits-of-recombinant-antibodies.png)
Anti-ALKBH2 antibody [EPR6177(2)] - BSA and Azide free (ab243917)
